Description
Aviva Systems Biology Prdx5 ELISA Kit (Mouse) (OKEH04367) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prdx5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed.After washing,abiotinylated detector antibody specific for Prdx5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Prdx5 captured in the well